Modified and Normal Cholinesterases in the Respective Strains of Carbamate-Resistant and Susceptible Green Rice Leafhoppers, Nephotettix cincticeps, UHLER : Hemiptera : Cicadellidae
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The modified cholinesterase, i.e., insensitive to toxicants, of the carbamate-resistant green rice leafhopper, was found to be separated from the normal cholinesterase of the susceptible hopper by DEAE-cellulose column chromatography. The enzymes of the resistant and susceptible strains were eluted in a single peak at the position of approximately 200,000 molecular weight on Sepharose 6B gel chromatography. On DEAE-cellulose chromatography the enzymes were eluted also in a single peak but the enzyme of the resistant strain was eluted at 0.2 M KCl faster than that of the susceptible strain at 0.25 M KCl.Thus, each enzyme of the two strains is chromatographically homogeneous, and could not be distriguished by Sepharose 6B gel, but could be clearly distinguished by DEAE-cellulose.These results suggest that the modified cholinesterase of the resistant strain may be a charge isomer of the normal one of the susceptible strain.
- 日本応用動物昆虫学会の論文
- 1976-09-25
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関連論文
- Sensitive Aliesterase to a Carbamate Insecticide, Propoxur, in the Resistant Strains of the Green Rice Leafhopper
- Insensitive Cholinesterase in the Nakagawara Strain of the Green Rice Leafhopper, Nephotettix cincticeps UHLER (Hemiptera : Cicadellidae), as a Cause of Resistance to Carbamate Insecticides
- Modified and Normal Cholinesterases in the Respective Strains of Carbamate-Resistant and Susceptible Green Rice Leafhoppers, Nephotettix cincticeps, UHLER : Hemiptera : Cicadellidae