Responses of ligated larvae of the flesh fly, Boettcherisca peregrina (Diptera : Sarcophagidae), to injected ecdysteroids : a possible usage for a simple in vivo assay for ecdysteroid measurement
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概要
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Mature larvae of the flesh fly, Boettcherisca peregrina, ligated at the tenth abdominal segment in the last larval stage showed a disturbance of puparium formation (pupariation) in the anterior portion. The anterior portion of the larval body was injected with samples tested. The disturbance of pupariation in the anterior portion was dose-dependently rescued by a single injection of ecdysteroids. The minimum effective doses of exogenous ecdysteroids to induce puparial tanning at the anterior portion were 125, 64, 64, 32, 16 and 8 ng for inokosterone, ecdysone, 20-hydroxy-ecdysone-22acetate, ponasterone A, 20-hydroxyecdysone and polypodine B, respectively. This assay showed a similar ecdysteroid-sensitivity to a conventional bioassay but produced results approximately 2 days earlier than the conventional assay that employed the isolated abdomen of B. peregrina larvae ligated at the sixth body segment.
- 日本応用動物昆虫学会の論文
- 2002-08-25
著者
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MORIBAYASHI Atsuko
Department of Medical Entomology, National Institute of Infectious Diseases
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AGUI Noriaki
Department of Medical Entomology, National Institute of Infectious Diseases
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Agui Noriaki
Department Of Medical Entomology National Institute Of Infectious Diseases
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Agui Noriaki
Department Of Medical Entomology National Institute Of Health
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Moribayashi Atsuko
Department Of Medical Entomology National Institute Of Infectious Diseases
関連論文
- Pupal diapause induction in larvae destined for non-diapause of the flesh fly, Boettcherisca peregrina : Diptera : Sarcophagidae
- The Role of Ecdysteroids and Juvenoids in Vitellogenin Levels and Follicle Development in the Housefly, Musca domestica
- Action of Juvenile Hormone on the Cerebral Neurosecretory Cells of Mamestra brassicae in vivo and in vitro
- Electrical Properties of the Cerebral Prothoracicotropic Hormone Cells in Diapausing and Non-Diapausing Pupae of the Tobacco Hornworm, Manduca sexta
- Ecdysteroid Titer and Its Critical Period during Larval and Pupal Ecdysis in the Cabbage Armyworm, Mamestra brassicae L. (Lepidoptera : Noctuidae)
- Responses of ligated larvae of the flesh fly, Boettcherisca peregrina (Diptera : Sarcophagidae), to injected ecdysteroids : a possible usage for a simple in vivo assay for ecdysteroid measurement
- Hemolymph Ecdysteroid Titers and Pupal Cuticle Synthesis in Mamestra brassicae
- Determination of Larval Instar Number in the Common Armyworm, Leucania separata (Lepidoptera : Noctuidae) : II. Timing of PTTH Secretion and Ecdysteroid Titer for Fifth Ecdysis
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