Discrimination of four Japanese Tetranychus species (Acari : Tetranychidae) using PCR-RFLP of the inter-transcribed spacer region of nuclear ribosomal DNA
スポンサーリンク
概要
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To establish diagnostic DNA markers useful for discriminating economically important Tetranychus species, especially T kanzawai, from other species of the urticae complex, we sequenced the ribosomal ITS region, including ITS 1, 5.8S, and ITS2, of four Tetranychus species and analyzed recognition sites of restriction endonucleases. We established genetic criteria for discriminating T kanzawai, T urticae, T pueraricola and T ludeni using PCR-RFLP. We propose the establishment of a worldwide PCR-RFLP catalogue.
- 日本応用動物昆虫学会の論文
- 2002-08-25
著者
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Osakabe Mh.
Department Of Plant Protection National Agriculture Research Center:(present Address)laboratory Of E
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Osakabe Mh.
Department Of Apple Research National Institute Of Fruit Tree Science
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Hirose Takuya
Kochi Prefectural Agricultural Research Center
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Sato Masaru
Yokohama Plant Protection Station
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Hirose Takuya
Kochi Prefectural Agricultural Research Center:(present Address)department Of Agriculture Forestry A
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Sato Masaru
Yokohama Plant Protection Station:(present Address)department Of Plant Protection National Agricultu
関連論文
- Discrimination of four Japanese Tetranychus species (Acari : Tetranychidae) using PCR-RFLP of the inter-transcribed spacer region of nuclear ribosomal DNA
- Phylogenetic analysis of green and red forms of the two-spotted spider mite, Tetranychus urticae Koch(Acari:Tetranychidae), in Japan, based on mitochondrial cytochrome oxidase subunit I sequences