Primary Culture of Developing Adult Tissue in a Nymphal Tick, Haemaphysalis longicornis(Acarina : Ixodidae)
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概要
- 論文の詳細を見る
Primary cultures of the developing adult tissues in nymphs of Haemaphysalis longicornis were described. All cultures were incubated at 25℃ in Leibovitz L-15 medium, pH 7.0,supplemented with 10% trytpose phosphate broth and 10% fetal bovine serum. The migrated cells from explants were of mostly fibroblast-like, epitherial-like or freely suspended type. They started to migrate within 24 hr after the culture was set up and formed cell networks by 30 days later. The cultures could be maintained for about 100 days. Subculturings of migrated cells, however, were unsuccessful and the cells began to degenerate resulting in remarkable reduction of their numbers after about 1 month.
- 日本応用動物昆虫学会の論文
- 1986-08-25
著者
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Fujisaki K
Obihiro Univ. Agriculture And Veterinary Medicine Hokkaido Jpn
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Mitsuhashi Jun
Division Of Entomology The National Institute Of Agricultural Sciences
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Mitsuhashi Jun
Division Of Entomology National Institute Of Agricultural Sciences
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Fujisaki Kozo
First Reaserch Division National Institute Of Animal Health
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KAMIO Tsugihiko
First Research Division, National Institute of Animal Health
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Kamio Tsugihiko
First Research Division National Institute Of Animal Health
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Mitsuhashi Jun
Division Of Entomology National Institute Of Agricltural Sciences:faculty Of Agriculture University
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Mitsuhashi Jun
Division Of Forest Protection Forestry And Forest Products Research Institute
関連論文
- Hemocyte Types and Their Primary Cultures in the Argasid Tick, Ornithodoros moubata MURRAY (Ixodoidea)
- Primary Culture of Developing Adult Tissue in a Nymphal Tick, Haemaphysalis longicornis(Acarina : Ixodidae)
- Isolation of a Continuous Cell Line from Larval Fat Bodies of an Arctiid Moth, Spilarctia seriatopunctata (Insecta, Lepidoptera, Arctiidae)(Cell Biology)
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- Melanization of Interguments Cultured in Vivo and In Vitro
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- Free Amino Acids in the Haemolymph of Some Lepidopterous Insects
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