Changes of Proteins and Nucleic Acids during Metamorphosis in the Midgut of the Silkworm, Bombyx mori L.(Lepidoptera : Bombycidae)
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概要
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Wet weight, total proteins, DNA and RNA of the midgut of hte silkworm, Bombyx mori, were analyzed to study the quantitative changes during metamorphosis. In addition, qualitative changes of soluble proteins were studied with polyacrylamide gel- and immunoelectrophoresis.During the larval-pupal transformation, the wet weight, total proteins and RNA of the epithelial midguts decreased almost linearly while DNA remained in abundance until just before larval-pupal ecdysis. After pupation, however, all components decreased to very small amounts by the time of eclosion. The lumenal contents of pupa and pharate adult contained a large amount of protein and DNA, whereas RNA was barely detectable.The midguts of male and female individuals did not differ quantitatively in protein and nucleic acid transformations at metamorphosis.Polyacrylamide gel- and immuno-electrophoretic analysis showed that the change in some protein components was stage-specific during holometabolous ontogeny; i.e., three kinds of protein could be differentigted, the larval, pupal and adult types. Both methods of electrophoresis also revealed that a great deal of proteins were common in larval and adult midguts, but differed greatly from those of the pupal midgut. The basis for the changes in protein components during the stage-specific protein synthesis at metamorphosis was discused from the point of view of gene switch-over and gene expression.
- 日本応用動物昆虫学会の論文
- 1981-05-25