Effect of Spore Sonication on Attachment and Host-Attachment Range of Pasteuria penetrans to the Root-Knot Nematode

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The optimum conditions for attachment to second-stage juveniles (J2s) of the root-knot nematode by Pasteuria penetrans sonicated spores, and their host-attachment range were studied. P. penetrans isolates MIP, MAP and MHP from Meloidogyne incognita, M. arenaria and M. hapla, respectively, were used. Spore attachment increased with increasing spore sonication time. Spores sonicated for 30 min had maximum attachment to J2, and attachment tended to decrease when spores were sonicated for 50 min. the increase in attachment with sonication was much higher in MIP than in MAP or MHP. When McIlvaine buffer of pH 4.0, 6.0 and 8.0 was used, the optimum pH for spore attachment shifted to a higher pH after spore sonication. The number of spores attached to J2 increased markedly with increases in temperature from 15 to 30℃. Each isolate of P. penetrans retained its host-attachment range after spore sonication. further, the sonicated spores of MAP, MIP MHP were examined for their host-attachment range among 10 Japanese Meloidogyne species. MAP attached in large numbers to only M. arenaria of both esterase types A-1 and A-2, and MIP to M. incognita, M, javanica and M. camelliae. However, MHP attached to a rather wide range of species, M. camelliae, M. hapla, M. mali, M. suginamiensis and a species close to M. mali.
1997-02-25