Phylogenetic utility of nucleotide sequences of mitochondrial 16S ribosomal RNA and cytochrome b genes in anthocorid bugs (Heteroptera:Anthocoridae)

概要

論文の詳細を見る
Nucleotide sequences of portions of the mitochondrial 16S ribosomal RNA gene (16S rDNA) and cytochrome b (cyt b) gene of eight species of the family Anthocoridae and of one outgroup taxon, Nezara viridula (Linnaeus), have been determined. From approximately 428-and 417-base pair aligned sequences of the 16S rDNA and cyt b gene, 185 and 171 variable sites were detected. As reported for some other insect groups, a strong A+T composition bias (78.3%) and A-T transversion bias(54.5% of all nucleotide substitutions) were detected in the 16S rDNA of anthocorid insects. However, in the cyt b gene, A-T transversions (29.2%) were less frequent than T-C transitions(39.8%), even though this gene also contained a relatively high level of A+T (70.7%). In the 16S rDNA, uncorrected genetic divergence increased linearly with an increase in taxonomic level. However, a plateau was observed in the values of the cyt b gene. This tendency was obvious in the third position of codons, in which the values increased very quickly and saturated at the between-subgenus level. These findings suggest that strong multiple substitutions occur in this position. Phylogenetic analyses showed that both the 16S rDNA and cyt b DNA fragments can be used to estimate relationships among anthocorid taxa, even though there are differences between them as regards taxonomic levels at which phylogenetic analysis can be applied.
日本応用動物昆虫学会の論文
2000-08-25