Novel Bombyx mori cell lines cultivable at 37℃

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概要

• 論文の詳細を見る

• Four continuous cell lines were established from cultures of embryonic tissue of the silkworm, Bombyx mori, and designated NISES-BoMo-MK, NISES-BoMo-KG, NISES-BoMo-DZ, and NISES-BoMo-OH. The cells were mostly spherical in shape, and could be cultured at 25℃ to 37℃. The population doubling time of each cell line was not affected by the temperatures employed, being about one day at both 25℃ and 37℃. The karyotype of the cells was typical of lepidopteran cell lines. The number of chromosomes was higher at 37℃ than at 25℃. The activity of the enzymes, phosphoglucose isomerase, phosphoglucomutase, isocitrate dehydrogenase, and glucose-6-phosphate dehydrogenase extracted from the cells was higher at 37℃ than at 25℃. None of these novel cell lines formed any polyhedra, even with high titer inoculation of Bombyx mori nuclear polyhedrosis virus.

• 日本応用動物昆虫学会の論文
• 1999-05-25

著者

• IMANISHI Shigeo

  Laboratory of Cell Engineering, National Institute of Sericaltunal and Entomological Science

• Cho E‐s

  Dong‐a Univ. Pusan Kor

• Cho Eun-sook

  College Of Natural Resources And Life Science Dong-a University

• Imanishi Shigeo

  Laboratory Of Cell Engineering National Institute Of Sericaltunal And Entomological Science

• Imanishi Shigeo

  Laboratory Of Cell Engineering Department Of Insect Genetics And Breeding National Institute Of Seri

• Tomita Shuichiro

  Developmental and Differentiation Department, National Institute of Agrobiological Science

• Tomita Shuichiro

  Laboratory of Cell Engineering, Department of Insect Genetics and Breeding, National Institute of Se

• Tomita S

  Developmental And Differentiation Department National Institute Of Agrobiological Science

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• Novel Bombyx mori cell lines cultivable at 37℃

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