Demonstration of interbreeding between virulent and avirulent populations of Bursaphelenchus xylophilm (Nematoda: Aphelenchoididae) by PCR-RFLP method

スポンサーリンク

概要

• 論文の詳細を見る

• The pinewood nematode, Bursaphelenchus xylophilus, is the causative agent of pine wilt disease. The virulence of nematodes significantly varies among pine trees, but does not vary within a single pine. To elucidate the reason for no variation in virulence within a single pine, a technique to investigate the population structure of nematodes of different virulence is needed. In this study, the demonstration of interbreeding between virulent and avirulent populations of B. xylophilus in vitro was attempted using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method. Ribosomal DNA containing the 5.8S gene, the internal transcribed spacer regions 1 and 2, and partial regions of 18S and 28S genes were used for analysis. First, PCR-RFLP patterns of offspring produced by interbreeding between two individuals of virulent and avirulent isolates were analyzed. The offspring from a single-pair interbreeding showed 3-digested fragment patterns with the restriction enzyme HhaI, patterns the same as the virulent isolate, the avirulent isolate and a hybrid pattern containing both fragments of the virulent and avirulent isolates. The virulent population was mixed with the avirulent one in vitro and propagated nematodes were individually analyzed by PCR-RFLP method. The nematodes showed the same 3 PCR-RFLP patterns as the offspring from a single-pair interbreeding. The detection of nematodes with a hybrid pattern demonstrates the occurrence of interbreeding between virulent and avirulent populations of B. xylophilus.

• 日本応用動物昆虫学会の論文
• 2003-11-25

著者

• Kikuchi Taisei

  Forestry and Forest Products Research Institute

• AIKAWA Takuya

  Forestry and Forest Products Research Institute

• KOSAKA Hajime

  Forestry and Forest Products Research Institute

• Kosaka H

  Forestry And Forest Products Research Institute

• Aikawa T

  Forestry And Forest Products Research Institute

• Aikawa Takuya

  Forest And Forestry Research Products Institute

関連論文

• Purification and Characterization of Recombinant Endoglucanases from the Pine Wood Nematode Bursaphelenchus xylophilus
• Purification and Characterization of Recombinant Endoglucanases from the Pine Wood Nematode Bursaphelenchus xylophilus
• Demonstration of interbreeding between virulent and avirulent populations of Bursaphelenchus xylophilm (Nematoda: Aphelenchoididae) by PCR-RFLP method
• Bursaphelenchus mucronatus (Nematoda : Aphelenchoididae) vectored by Monochamus urussovi (Coleoptera : Cerambycidae) in Hokkaido, Japan
• An inoculation experiment of Japanese Bursaphelenchus nematodes on Japanese black and red pine, Pinus thunbergii and P. densiflora

スポンサーリンク