Discrimination of Japanese isolates of Beauveria brongniartii (Deutermycotina: Hyphomycetes) by RFLP of the rDNA-ITS regions
スポンサーリンク
概要
- 論文の詳細を見る
Isolates of the entomopathogenic fungus Beauveria brongniartii differ in the shape of the conidia and the amount of red pigment production, which are associated with the host insects they were obtained from. We investigated intraspecific polymorphisms among 38 isolates of B. brongniartii by using restriction fragment length polymorphisms (RFLP) of the internal transcribed spacer regions (ITS1 and ITS2), 5.8S rDNA, and small portions of the 18S and 28S rDNAs. Based on the RFLP patterns, the isolates were divided into two distinct groups. One group was composed mainly of isolates from longicorn beetles (Coleoptera: Cerambycidae), whereas the other group mainly contained isolates from scarab beetles (Coleoptera: Scarabaeidae). The DNA sequences of the amplified regions showed high homology between the groups, with only 7 substitutions in 852 nucleotides, andjust 1 of the 7 substitutions accounted for the difference in the RFLP patterns between the two groups. Furthermore, RFLP successfully distinguished all of the B.brongniartii isolates from the 5 tested isolates of B. bassiana and the 1 of B. amorpha.
- 日本応用動物昆虫学会の論文
- 2003-11-25
著者
-
HORITA Mitsuo
National Agricultural Research Center for Hokkaido Region
-
SHIMAZU Mitsuaki
Forestry and Forest Products Research Institute
-
Horita M
National Agricultural Research Center For Hokkaido Region
-
WADA Sanae
National Institute of Agrobiological Sciences, Insect and Animal Sciences Division
-
HIRAYAE Kazuyuki
National Agricultural Research Center for Kyushu Okinawa Region
-
Wada S
National Institute Of Agrobiological Sciences Insect And Animal Sciences Division
-
Wada Sanae
National Institute Of Agrobiological Sciences
-
Shimazu Mitsuaki
Forestry And Forest Products Res. Inst.
-
Hirayae K
National Agricultural Research Center For Kyushu Okinawa Region
-
Wada Sanae
National Institute For Sericultural And Entomological Science
関連論文
- Media for conidial production of the entomopathogenic fungus, Fusarium coccophilum (Anamorphic fungi: Phialosporae)
- Effects of larval age on mortality of Monochamus alternatus Hope (Coleoptera : Cerambycidae) after application of nonwoven fabric stripa with Beauveria bassiana
- Analysis of sources of oxolinic acid-resistant field strains of Burkholderia glumae based on rep-PCR analysis and nucleotide sequences of gyrB and rpoD
- Granulovirus-infected larvae of Pseudaletia separata (Lepidoptera : Noctuidae) produce factor(s) toxic to an endoparasitoid, Cotesia kariyai (Hymenoptera : Braconidae)
- Isaria javanica (anamorphic Cordycipitaceae) isolated from gypsy moth larvae, Lymantria dispar (Lepidoptera: Lymantriidae), in Japan
- Effects of temperature on growth of Beauveria bassiana F-263, a strain highly virulent to the Japanese pine sawyer, Monochamus alternatus, especially tolerance to high temperatures
- Density dynamics of the entomopathogenic fungus, Beauveria bassiana Vuillemin (Deuteromycotina: Hyphomycetes) introduced into forest soil, and its influence on other soil microorganisms
- Density of the entomopathogenic fungus, Beauveria bassiana Vuillemin (Deuteromycotina: Hyphomycetes) in forest air and soil
- Aerial Infection of Cordyceps militaris LINK (Clavicipitales: Clavicipitaceae) against Larvae of Quadricalcanfera punctatella (MOTSCHULSKY) (Lepidoptera: Notodontidae)
- Detection of Cordyceps militaris LINK (Clavicipitales: Clavicipitaceae) by Burying Pupae of Quadricalcarifera punctatella MOTSCHLSKY (Lepidoptera: Notodontidae)
- Persistence of Metarhizium anisopliae(Deuteromycotina:Hyphomycetes)as a Control Agent of Anomala cuprea(Coleoptera:Scarabaeidae)in a Forestry Nursery
- Control of Epinotia granitalis(Lepidoptera:Tortricidae)with Paecilomyces spp. on Cotton Bands Wrapped on the Trunks of Cryptomeria japonica
- Relation of Japanese Xanthomonas campestris pv. vesicatoria with worldwide strains revealed with three specific monoclonal antibodies
- Spongospora subterranea soil contamination and its relationship to severity of powdery scab on potatoes
- Purification and Properties of a New Chitin-binding Antifungal CB-1 from Bacillus licheniformis
- Discrimination of Japanese isolates of Beauveria brongniartii (Deutermycotina: Hyphomycetes) by RFLP of the rDNA-ITS regions
- Stromata production for Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects
- Microbial Control of Monochamus alternatus HOPE (Coleoptera: Cerambycidae) by Application of Nonwoven Fabric Strips with Beauveria bassiana (Deuteromycotina: Hyphomycetes) on Infested Tree Trunks
- Stability of the viral-enhancing ability of entomopoxvirus spindles exposed to various abiotic factors
- Comparative Analysis of Japanese and Foreign Strains of Ralstonia solanacearum Based on 16S Ribosomal RNA Gene Sequences
- Media for Selective Isolation of an Entomogenous Fungus, Beauveria bassiana (Deuteromycotina: Hyphomycetes)
- Differences in the susceptibility among silkworm, Bombyx mori (Lepidoptera : Bombycidae), strains to the entomopathogenic fungus, Beauveria brongniartii and the mode of inheritance of the susceptibility
- Infectivity of Hibernated Resting Spores of Entomophaga maimaiga HUMBER, SHIMAZU et SOPER (Entomophthorales : Entomophthoraceae)
- Metarhizium cylindrosporae CHEN et GUO (Deuteromycotina : Hyphomycetes), the Causative Agent of an Epizootic on Graptopsaltria nigrofuscata MOTCHULSKI (Homoptera : Cicadiae)
- PCR-based specific detection of Ralstonia solanacearum race 4 strains
- Phylogenetic relationships of Ralstonia solanacearum species complex strains from Asia and other continents based on 16S rDNA, endoglucanase, and hrpB gene sequences
- Effect of Rearing Humidity of Host Insects on the Spore Types of Entomophaga maimaiga HUMBER, SHIMAZU et SOPER : Entomophthorales : Entomophthoraceae
- Isolation of an entomogenous fungus, Erynia delphacis (Entomophthorales : Entomophthoraceae), from migratory planthoppers collected over the Pacific Ocean
- Analysis of genetic and biological characters of Japanese potato strains of Ralstonia solanacearum
- Introduction of Entomophthora muscae (Zygomycotina: Entomophthorales) to Caged House Fly Populations
- Evaluation of House Fly Cadavers as Conidial Sources for Introduction of the Parasitic Fungus Entomophthora muscae (Zygomycotina: Entomophthoraceae)
- A refined inoculation method to evaluate false smut resistance in rice
- Sequencing Result of 16S, HrpB, and Endoglucanase Genes widen the Diversity of Asian Strains of Ralstonia solanacearum(The Annual Meeting of the Society in 2003)
- Bacterial Wilt of Zingiberaceae Plants Caused by Ralstonia solanacearum Race 4 strains in Japan(The Annual Meeting of the Society in 2003)
- Genetic Diversity of Zingiberaceae Plant Isolates of Ralstonia solanacearum in the Asia-Pacific Region
- Linkage analysis of a dominant resistance gene to the entomopathogenic fungus Beauveria brongniartii in the silkworm, Bombyx mori