Presence of alternative complement pathway-inhibiting protein (ACPIP) from silkworm (Bombyx mori): changes in activity at various stages of fifth larval stadium

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概要

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• The activities of human alternative complement pathway-inhibiting protein (ACPIP) were examined in silkworm (Bombyx mori) larvae at various developmental stages, using both hemolysis-inhibition plate assay and immunoblotting analysis. Significant activities were detected in the hemolymph ranging from the fourth rnolt to prepupal stages, the highest being those on the 6th to 9th days of the fifth stadium. Although the activity was also detected at the pupal stage, it was only 1/16 of the maximum. These results suggested that ACPIP in the silkworm hemolymph might play a physiological role at the larval stage rather than at the pupae stage. ACPIP-like activities were also detected in hemolymph from final stadium larvae of Antheraea pernyi, Hense convolvuli, Antheraea yamamai, and Rhodinia fugax.

• 2003-05-25

著者

• Hiyama Tetsuo

  Department of Biochemistry, Faculty of Sciences, Saitama University

• Hamano Kunikatsu

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology

• Hamano Kunikatsu

  Department Of International Environmental And Agricultural Science Graduate School Of Agriculture

• Hamano Kunikatsu

  Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn

• Sekijima Y

  Department Of Immunology Saitama Prefectural University Junior College

• Hiyama Tetsuo

  Department Of Biochemistry Faculty Of Sciences Saitama University

• Hiyama T

  Department Of Biochemistry And Molecular Biology Saitama University

• FUJIKURA Yuriko

  Department of Immunology, Saitama Prefectural University, Junior College

• SEKIJIMA Yasutaka

  Department of Immunology, Saitama Prefectural University, Junior College

• CHENG Zhang

  Department of Biotechnology, Shenyang Agricultural University

• SAITO Jun

  Development and Differentiation Department, National Institute of Agrobiological Science

• TAKAHASHI Takashi

  Nakasato Group for Wild Silk Moths Rearing

• Fujikura Y

  Department Of Immunology Saitama Prefectural University Junior College

• Hamano K

  Department Of Biological Production Tokyo University Of Agriculture And Technology

• Cheng Zhang

  Department Of Biotechnology Shenyang Agricultural University

• Saito J

  Pasco Corporation

• Takahashi T

  Nakasato Group For Wild Silk Moths Rearing

• Hiyama Tetsuo

  Department of Biochemistry and Molecular Biology, Saitama University

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