Porphyromonas endodontalisの病原因子の解析
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概要
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Porphyromonas endodontalis, a gram-negative anaerobic rod, is an important pathogenic organism in periapical lesions with acute symptoms, such as pain, swelling, and suppuration in endodontic patient. Like P. gingivalis, a major pathogen of adult periodontitis, P. endodontalis is asaccharolytic and forms black-pigmented colonies on enriched blood agar plates. However, pathogenic factors and pathological potential of this microbe have been poorly characterized. In this study, we assessed the adhesive abilities of P. endodontalis to gingival epithelial cell, and investigated whether P. endodontalis has a major fimbrial component of fimbrilin similar to that of P. gingivalis. Furthermore, the proteolytic activities of P. endodontalis were examined. The results indicated that P. endodontalis significantly adhered to a mouse gingival epithelial cell line, GE1, although the adhesive ability was lower than that of P. gingivalis. The electron microscopic investigations showed scarcely any fimbriae in P. endodontalis. Furthermore, PCR and the DNA-DNA hybridization analysis for the gene encoding fimbrilin (fimA) of P. gingivalis revealed that P. endodontalis did not have fimA or fimA-related genes, which could account for the lesser adhesive ability of P. endodontalis to gingival epithelial cells. The hydrolyzing profile toward various synthetic peptides showed that the spectrum of proteolytic activities of P. endodontalis was markedly different from that of P. gingivalis. Thus, the present findings indicate that P. endodontalis has a different adhesion mechanism from P. gingivalis fimbriae, and has a different spectrum of proteolytic activities from that of P. gingivalis, which may lead to the specific pathological potential of the organisms in periapical lesions in humans.
- 岩手医科大学歯学会の論文
- 2002-12-24