The Effects of Methylated Cyclodextrins on the Transphosphorylation among AMP, ADP and ATP in the Presence of Escherichia Coli
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概要
- 論文の詳細を見る
Transphosphorylation, 2ADP⇄AMP+ATP, occurred in the presence of intact Escherichia coli (E. coli. strain K-12) in neutral phosphate buffer solution, because adenylate kinase existed in both cytoplasma and periplasma of E. coli. Cyclodextrin(CD)s accelerated the transphosphorylation. The reaction rate depended on the concentration of CD and the kind of CD. Methylated CDs were more effective than parent CD, especially, hiptakis-(2,6-di-O-methyl)-β-CD(DMCD) was most effective. Addition of CDs makes neither change of E. coli concentration nor the leak of adenylate kinase from periplasma of E. coli.
- 東京工芸大学の論文
- 1992-01-31
著者
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Shimizu Toshihiko
The Faculty Of Engineering Tokyo Institute Of Polytechnics
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Hattori K
Tokyo Inst. Polytechnics Kanagawa Jpn
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TAKAHASHI Keiko
The First Department of Internal Medicine, Hyogo College of Medicine
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Takahashi K
Tokyo Inst. Polytechnics Kanagawa Jpn
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SONE Hironobu
The Faculty of Engineering Tokyo Institute of Polytechnics
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HATTORi Kenjiro
The Faculty of Engineering Tokyo Institute of Polytechnics
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Takahashi Keiko
The Faculty Of Engineering Tokyo Institute Of Polytechnics
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- The Effects of Methylated Cyclodextrins on the Transphosphorylation among AMP, ADP and ATP in the Presence of Escherichia Coli
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