RESPIRATION AND OXIDATIVE PHOSPHORYLATION OF MITOCHONDRIA FROM TISSUES AND ORGANS OF ANTARCTIC FISH (18th Symposium on Polar Biology)

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概要

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• Research has been carried out on the effect of temperature on the physiological properties of mitochondria isolated from liver, cardiac muscle and encephalon of the Antarctic fish Notothenia neglecta. Mitochondria were obtained in a medium containing 0.25 M mannitol, 0.075 M sucrose, 10^<-3> M EDTA, and 10^<-2> M TRIS, in 10^<-3> M phosphate buffer, pH 7.4. All assays were carried out by means of a polarograph assembled with an oxygen electrode possessing a temperature sensor. Respiration rates, oxidative phosphorylation and respiratory control values were assayed in regard to the effect of temperature. All preparations of liver, cardiac muscle and encephalon mitochondria displayed a very efficient respiration mechanism at 1℃. No general pattern for the influence of temperature increase has been found in those preparations. On the other hand, encephalon mitochondria displayed an efficient system for ATP generation by oxidative phosphorylation, but sensitive to temperature increase.

• 国立極地研究所の論文

著者

• Bacila Metry

  Universidade Federal Do Parana Laboratorio De Piscicultura

• Sugizaki Mateus

  Faculdade De Ciencias Universidade Estadual Paulista

• Malucelli Maria

  Institute Butantan Divisao De Desenvolvimento Tecnologico E Producao

• LUCCHIARI Pedro

  Centro de Ciencias Biologicas e da Saude, Pontificia Universidade Catolica do Parana

• Lucchiari Pedro

  Centro De Ciencias Biologicas E Da Saude Pontificia Universidade Catolica Do Parana

関連論文

• RESPIRATION AND OXIDATIVE PHOSPHORYLATION OF MITOCHONDRIA FROM TISSUES AND ORGANS OF ANTARCTIC FISH (18th Symposium on Polar Biology)

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