EFFECTS OF INCUBATION TEMPERATURE ON DISTRIBUTION OF ^<14>C IN LIPIDS IN A PSYCHROPHILIC MARINE DIATOM (Twelfth Symposium on Polar Biology)

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The incorporation of [14C] bicarbonate into cells and the lipid fractions was determined at 6°, 16° and 26℃ using the psychrophilic marine diatom, Navicula sp. strain D grown at 3℃ and 13℃. The intramolecular distribution of ^<14>C in both total lipids and three lipid classes (phospholipids, glycolipids and neutral lipids) was also estimated. The rates of ^<14>C incorporation into cells and the total lipid fraction were highest at 16℃ followed in order by those at 6℃ and 26℃. The percentage of total incorporated ^<14>C present as total lipids was significantly low at 26℃ compared with those at 6℃ and 16℃. Among the three lipid classes, neutral lipids were predominantly labeled (50-80%) under the conditions employed. Relative radioactivity of total lipids localized in fatty acid methyl esters was significantly lower at 26℃ than at 6℃ and 16℃. This decrease at 26℃ was attributable to a relative decrease of phospholipids and neutral lipids, the radioactivities of which were heavily presented in acyl moieties. These changes at 26℃ were more remarkable with cells grown at 3℃ than with those grown at 13℃.
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