Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス誘導における一酸化窒素の関与

概要

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Nitric oxide (NO) produced by activated macrophages is known to have antibacterial effects and to act as an inflammatory modulator. The aim of this study was to determine whether infected macrophages produce NO after Actinobacillus actinomycetemcomitans infection, and whetherthe produced NO is involved in regulating subsequent apoptosis. The expression of inducible NO synthetase (iNOS) was examined. In addition, culture supernatant was obtained to measure NO levels and LDH activity in the presence and absence of S-methylisourea (SMT), a specific inhibitor of iNOS. Celluler protein was extracted from infected macrophages to measure DNA fragmentation and caspase activity. The NO levels were increased by the infection. The LDH activity, DNA fragmentation, and caspase activity were also increased by the infection, and increased further with addition of SMT. These findings indicate that infected macrophages produce NO to protect themselves against apoptotic cell death by decreasing caspase activity.
北海道医療大学の論文
2001-12-31