LECTION BINDING PROFIELS IN NORMAL LUNG ERITHELIAL CELLS AND LUNG CANCERS: PROGNOSTIC SIGNIFICANCE OF VICIA VILLOSA REACTIVITY

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Carbohydrate expression in normal lung epithelia has not been well delineated, and its clinicopathologic significance in lung cancer remains to be elucidated. Using 14 peroxidase-labeled lectins, we studied 5 normal autopsied lungs with an appropriate fixation and 50 primary lung tumors with an ample follow-up period. Each type of normal epithelial cells showed different patterns in lectin reactivity, which was confirmed by lectin-gold silver techniques. The results were compared with those in the previous studies, and consistent lectin binding profiles were summarized. The profiles of goblet cells of the surface epithelium and mucous cells of the bronchial glands were considerably different, the latter profile in a strong contrast with that of serous cells. Clara cells and type II cells shared binding of similar lectins except negative Dolichos biflorus agglutinin (DBA) in the latter. The lectin binding patterns were rather uniform in 31 adenocarcinomas of various subtypes. These tumors showed a high positive rate (>50% of cases) in reactivity with Canavalia ensiformis (Con A), Lotus tetragonolobus (Lotus), Arachis hypogaea (PNA), DBA, Glycine maximus (SBA), Solanum tuberosum (STA), and Triticum vulgaris (WGA), a diverse profile different from those in any of the normal lung epithelial cells. Ten squamous cell carcinomas generally showed a lower positive rate with each lectin, but the binding profile was not significantly different from that in adenocarcinoma. Five large cell carcinomas showed much less reactivity, but still retained a similar pattern. Four small cell carcinomas showed positivity only with Con A and WGA. These results, generally compatible with those of the previous studies, may indicate that carbohydrate expression is similar among non-small cell lung cancers (NSCLCs), but is different between NSCLC and small cell carcinoma. Vicia villosa (VVA) reactivity, not detected in the normal lung epithelia, was found in 13 adenocarcinomas (42%), and the VVA positivity correlated with a more frequent lymph node metastasis (p=0.037) and a shorter survival of patients (p=0.024). In multivariate analysis the VVA reactivity also emerged as an independent prognostic factor. This may be explained by the fact that VVA binds specifically to N-acetyl-galactosamine, also the terminal structure of Tn antigen, reported to confer an invasion and migration advantage on tumor cells.
名古屋市立大学の論文
2003-02-28

LECTION BINDING PROFIELS IN NORMAL LUNG ERITHELIAL CELLS AND LUNG CANCERS: PROGNOSTIC SIGNIFICANCE OF VICIA VILLOSA REACTIVITY

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