トロポミオシンペプチドを固相化抗原としたELISAの確立とこれを用いた潰瘍性大腸炎患者血清における杭トロポミオシンペプチド抗体の測定とその意義
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Autoimmune phenomena have been implicated in the pathogenesis of ulcerative colitis (UC). We have previously revealed that ulcerative colitis colon-bound IgG recognizes Mr. 40KD colonic protein, which belongs to the cytoskeletal protein-tropomyosins (TMs). If TMs do in fact play a role as auto-antigens in patients with UC, specific antibodies against TMs must be present in these patients. However, a sensitive and reproducible assay system to detect anti-TMs antibody has not been established, and the presence of anti-TMs antibody in UC has not been elucidated well. In patients with UC in Japan, a significant accumulation of HLA-DR2-DPw9 haplotype was noted by serological typing of HLA molecules. We have previously investigated the association of HLA class II molecules with TM peptides, and revealed that the tryptic peptide of TM did indeed bind to HLA-DPw9 but not to HLA-DR2. In the present study, we identified by computer homology analysis a specific peptide (HIAEDADRK) in human TM that can bind to HLA-DPw9, and established a sensitive and reproducible Enzyme linked immunosorbent assay (ELISA) using this synthetic peptide (HIAEDADRK) as a coating antigen. With this newly established peptide-based ELISA, anti-TM antibody was detected in sera of patients with UC and primary sclerosing cholangitis (PSC), which is known to be an extra-colonic manifestation of UC, with high reproducibility. This ELISA system may offer an useful tool to detect an anti-TM antibody for a diagnosis of UC.
- 札幌医科大学の論文
- 2000-04-01
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