ラット摘出心の虚血再灌流障害に対するANPの抑制効果についての検討

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Several reports have shown that the Atrial natriuretic peptide (ANP) protects the myocardium from ischemic and reperfusion injury. Since these studies were performed via the regional ischemia model, it is unclear whether the effect of ANP on the ischemic myocardium is a secondary effect by way of improved subendocardial blood flow or is due to some other direct effect of ANP. The present study aims to show that ANP has a direct cardioprotective effect in a global ischemia model of isolated rat heart. The constant flow perfusion method was performed using the Langendorff apparatus. Rat hearts were exposed to global ischemia for 30 min followed by reperfusion for 40 min. Left ventricular performance, myocardial energy status, release of creatine kinase and tissue water content were measured after reperfusion. Ultrastructural study was performed in four groups (n=5). Group C consisted of untreated normal rat hearts which underwent pre- and postischemia in Krebs Henseleit bicarbonate buffer (KHB) solution. Groups H1, H2 and H3 were all treated with a KHB+ ANP 100 nmol/l solution during preischemic, postischemic and pre- and postischemic periods respectively. The hearts from the H3 group demonstrated the best myocardial protection on ischemic and reperfusion injury among the 3 treated groups. The recovery of cardiac function and the reduction of ischemic tissue damage in the H1 group were significantly better than in the C group. However there was no significant difference in the myocardial damage between the C and H2 groups, the recovery of cardiac function in the H2 group was significantly better than in the C group. The present study shows that treatment with ANP during pre- and postischemia appears to be effective in preventing ischemic and reperfusion injury through direct myocardial protection. These data indicate that the treatment with ANP during preischemic period protects the myocardium from irreversible ischemic damage and the treatment with ANP during postischemic period prevents the occurrence of the myocardial stunnig after reperfusion.
札幌医科大学の論文
2000-04-01

ラット摘出心の虚血再灌流障害に対するANPの抑制効果についての検討

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