Evans blue 染色によるラット脳内腫瘍同定方法の検討及び臨床応用
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概要
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We established a method of intraoperative labeling of intracerebral tumors by using a biological dye, which has been used as an indicator of the disruption of the blood-brain barrier incurred in such disorders as cerebral tumors. Intracerebrally transplanted tumors in rats were stained successfully with Evans blue (EB) dye, which was a useful indicator of tumor location and its margins. EB at 100 mg/kg i. v. administration was enough to stain the tumors without any acute toxic effects ; however, the whole body of rat including the internal organs were also stained blue. EB dose at lower than 10 mg/kg could eliminate the skin and eye discoloration, and the dose could be reduced to as low as 1.25 mg/kg to get the recognizable tumor staining. The staining continued for at least 96hrs after the injection. The greatest difference in the dye concentration between serum and tumor tissue was achieved at 24 hrs after the injection, when the serum concentration showed a 38% decrease from its maximum value. The threshold level of the tissue dye concentration for visual recognition was 50 pg/g of tumor tissue. Histological specimens examined after the stained tumors (1.25 mg/kg i. v.) removal with an operative microscope showed complete tumor removal except for a tiny portion of an infiltrated area. Operators found it easier to identify and resect the stained tumors than unstained ones. From April 1997 to May 1998, we used the EB dye for identifying intracerebral tumors on 3 patients. Two out of the 3 patients apparently showed blue discoloration of the tumors, which were identifiable during the operations, and the operators found this method helpful for resection, The 2 tumors which stained positive showed tissue dye concentrations of more than 50 fig/g, and the other one with negative staining showed a tissue concentration of below 50 pg/g; this data conformed very well with that obtained from the animal data. We speculated that a rather long-term administration of steroids before operation could interfered with the perme-ation of EB dye though the blood-brain barrier.
- 1998-08-01