Efficiency-Monitored Representational Difference Analysis による若年者胃癌からのEpstein-Barr ウイルスの単離
スポンサーリンク
概要
- 論文の詳細を見る
Gastric cancer in young adults is one of the most mortal neoplasms and is characterized by histopathological features of poorly differentiated type and infiltrative growth. However, very little is known about the genetic mechanisms of this malignant tumor, which is difficult to analyze due to the proliferation of scirrhous stroma. The aim of this study was to apply an improved representational difference analysis (RDA) in order to identify the genetic alterations in gastric cancer of young adults. To monitor the efficiency of RDA, mouse DNA fragment was added to a tester prepared from the genomic DNA of gastric cancer of a 24-yr-old woman, at the rate of a single copy per haploid genome (efficiency-monitored RDA (EM-RDA)). The difference products were analyzed by Southern blot with the mouse DNA as a probe. The real difference products, other than the mouse DNA, were subcloned and sequenced. As a result, added control DNA fragments were sufficiently amplified through 3 rounds of subtraction and the high efficiency of the subtraction was confirmed. Another two difference clones were identified as DNA fragments of Epstein-Barr virus (EBV). The existence of EBV in the gastric cancer cells was proved by EBV-encoded small RNA 1 in situ hybridization. In summary, efficiency-monitored RDA was demonstrated to raise the reliability of RDA. Furthermore, this method successfully contributed to the detection of EBV in the gastric cancer of a young woman.
- 札幌医科大学の論文
- 1997-12-01
著者
関連論文
- BOOP病変が先行した抗Jo-1抗体陽性多発性筋炎の1例
- 巨大な骨盤腔内腫瘤を伴った慢性骨髄性白血病に施行した同種骨髄移植
- Differential display 法を用いたG2/M期に関連する遺伝子の解析
- S状結腸に穿破した急性虫垂炎の1例
- 肺結核の加療中に発症した続発性副腎機能不全の1例
- Addison病を併発した副腎原発悪性リンパ腫
- Efficiency-Monitored Representational Difference Analysis による若年者胃癌からのEpstein-Barr ウイルスの単離
- Representational Difference Analysis (RDA) 法によるスキルス胃癌での異常DNA断片単離と解析
- Differential display法を用いたG2/M期に関連する遺伝子の解析