LOCALIZATION OF PROTEOGLYCANS IN THE CELL NUCLEUS OF SEA URCHINS AS REVEALED BY MEANS OF LECTIN-BINDING AND NUCLEASE DIGESTION

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概要

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• The distribution of proteoglycans in the embryonic cell nucleus of sea urchins was studied by means of lectin binding using Ricinus communis agglutinin I (RCA-120) and other lectins. By electron microscopy using avidin-biotin complex technique, the localization of nuclear proteoglycans was found to be restricted exclusively to areas of dispersed chromation, i.e. euchromatic regions, in the uncleus. Fluorometric determination of lectin binding ability onto the partially digested chromatin using FITC-labeled lectins showed that lectin binding sites were exposed by the action of pancreatic DNase-I but remained masked by Staphylococcal nuclease. Considering the difference of the mode of action of these enzymes, it is likely that the lectin binding sites are more abundant in the regions of active genes than those of inactive ones. The pattern of localization of the nuclear proteoglycans suggests a possible participation of these substances in the mechanism of gene activation.

• 埼玉医科大学の論文
• 1998-03-31

著者

• KINOSHITA S.

  Department of Physics, Tohoku University

• Yoshii K.

  Nursing School St. Marianna University School Of Medicine

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• LOCALIZATION OF PROTEOGLYCANS IN THE CELL NUCLEUS OF SEA URCHINS AS REVEALED BY MEANS OF LECTIN-BINDING AND NUCLEASE DIGESTION

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