<原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与

概要

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This study investigated the involvement of caspase in apoptotic cell death of A. actinomycetemcomitans-infected macrophages. Two peptide inhibitors of caspases, Z-VAD-FMK and Z-DEVD-FMK, inhibited apoptotic cell death of J774.1 infected with A. actinomycetemcomitans. During the process of apoptosis, IL-1β was detected in the culture supernatants of J774.1 cells. The IL-1β secretion was blocked by the caspase-1 inhibitor, Z-VAD-FMK. Immunoblot analysis showed that the infection of A. actinomycetemcomitans to J774.1 cells induced the cleavage of Rb, suggesting that caspase-3 was activated by A. actinomycetemcomitans-infection. The cytosol from A. actinomycetemcomitans-infected J774. 1 cells induced Rb proteolysis in vitro, which was inhibited by the caspase-3 inhibitor, Z-DEVD-FMK. Furthermore, caspase-3-like activity was markedly increased in J774.1 cells infected with A. actinomycetemcomitans between 12h and 24h, which was subsequently inhibited by the addition of Z-VAD-FMK and Z-DEVD-FMK. These findings indicate that caspase-3 induces apoptosis in J774.1 cells infected with A. actinomycetemcomitans. Taken together, these results suggest that caspase-1 and caspase-3 are involved in the induction of apoptosis in A. actinomycetemcomitans-infected macrophages.
北海道医療大学の論文
2001-06-30