<ORIGINAL ARTICLE>ESR spin trapping study on ascorbic acid as a scavenger of hydroxyl and hydrogen radicals

概要

論文の詳細を見る
The present study was carried out to confirm the usefulness of the ESR spin trapping technique for detecting and measuring short-lived radicals and to clarify the ability of ascorbic acid as a radical scavenger against radicals generated from irradiated human serum. One hundred μl of human serum was irradiated with 1 Gy of X-ray radiation coexisting with 35 μl of DETAPAC, 50 μl of various concentrations (2×10^<-4>M〜60×10^<-4>M) of ascorbic acid, and 15 μl of DMPO. After irradiation at each concentration, the ESR signals were recorded using an ESR spectrometer. These signals were identified, and then normalized as the relative signal intensities against the standard signal intensity of manganese oxide marker. The relative signal intensities were plotted, and the results showed that the signals were from DMPO-OH and DMPO-H, showing the generation of ・OH and ・H, and that the relative signal intensities obtained at each concentration decreased as the concentration of ascorbic acid increased.
北海道医療大学の論文
1997-12-30