<原著>ラット耳下腺腺房細胞の開口分泌過程における細胞骨格の形態学的研究

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概要

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• In the present study, the investigation on the role that cytoskeleton may play in exocytosis which were enzymatically dispersed rat parotid acini has been approached by morphological techniques. The auther examined microfilaments, intermediate filaments and microtubules by immunofluorescence technique and electron microscopic observation. Furthermore, distribution of microfilaments using heavy meromyosin (HMM) decoration method was observed. The results obtained were as follows 1 In response to secretory stimulation with isoproterenol (IPR) or carbachol (CCH), parotid acini revealed exocytotic secretion that lumen was dilated and the number of secretory granules had markedly decreased. 2 Immunohistochemistry showed that actin was localized in the granular pattern of staining with the cytoplasm. Microfilament stained with rhodamine-phalloidin, which binds selectively to polymerised actin filaments. Most of the microfilaments were localized beneath the apicalplasma membrane. Upon stimulation with isoproterenol (IPR), the number of individed rhodamme phalloidm positive small granules increased dramatically, indicating that the secretory granules was surounded by microfilaments after the fusion with luminal membrane. 3 Intermediate filaments were stained by immunofluorescent techique using antibodies against cytokeratin, while microtubles were stained with anti β-tubulm In acinar cells, the intermediate filaments exhibited a reticular distribution in the cytoplasm. The microtubules exhibited aradial distribution in cytoplasm of longitudially from the apical resion to the basal resion. At secretory phase, the distribution of intermediate filaments and microtubules in the acinar cells were almost the same as the resting phase. 4 Ultrastructurally, microfilaments were not detectable around the secretory granules which stored in the cytoplasm, but were observed around them whose membranes were continuous with th luminal plasma membrane during exocytosis. These results indicate that microfilaments are localized mainly underneath the luminal plasma membrane at the resting phase and act as blockade to exocytosis. At the secretory phase, microfilaments allow exocytosis by encerclmg the discharged secretory granule membranes, provide forces for the extrusion of the secretory products through the action of actm-myosin contractile system.

• 北海道医療大学の論文
• 1996-12-31

著者

• 佐々木 泰裕

  北海道医療大学歯学部

関連論文

• ラット耳下腺腺房細胞の開口分泌過程における細胞骨格の形態学的研究
• 19.耳下腺アミラーゼ開口分泌における細胞骨格系の関与について(東日本学園大学歯学会第10回学術大会(平成4年度))
• 4.ラット耳下腺におけるフォドリンの役割(第9回東日本学園大学口腔外科研究会)

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