Dengue virus type 2 unresponsive to the current PCR primer; : construction of a new PCR primer to detect all strains of Dengue virus type 2.
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概要
- 論文の詳細を見る
We found that one strain of dengue virus (Trinidad 1751; TR) did not respond to the PCR primer for Jamaica/83. We investigated such property with other 10 strains of dengue virus type 2 and found 2 more unresponsive strains. All 3 strains were isolated from the central America. To detect the envelope gene of those 3 strains by PCR, we synthesized primers based on TR strain as the reference sequence. Using these primers, we could detect the 3 strains by PCR at the usual annealing temperature. We recommed the new primer for diagnosis of DEN 2.
- 長崎大学の論文
著者
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Kobayashi Masami
Department of Global Environmental Engineering, Kyoto University
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Hirota Noriko
Department Of Virology I National Institute Of Health
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Kameoka Y
National Inst. Infectious Diseases Tokyo Jpn
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Kameoka Yosuke
Division Of Genetic Resource National Institute Of Infectious Disease
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Kobayashi Masami
Department Of Dermatology National Defense Medical College
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Kobayashi Masami
Department Of Virology I National Institute Of Health
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Kobayashi Masami
Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama-Gakuin University
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