陽イオン交換分離法によるモノグアニルメラニンの定量法

概要

論文の詳細を見る
An ion-exchange chromatographic technique for the separation of monoguanylmelamine by means of a stepwise elution from a 0.8φ×20 cm column of a strongly acidic cation exchange resin, Dowex 50WX4,in the sodium form is studied. As the eluant, Sφrensen's buffer solution consisting of glycine, NaOH and NaCl is used. An aqueous solution of the sample is passed through the column. At first, interfering substances are eluted with pH 9.9∿10.0 buffer solution. The elution is continued until the absorption of the effluent at 255 nm is not appear. About 250 ml of the eluant is necessary for this. Then, monoguanylmelamine is eluted with pH 11.1∿11.5 buffer solution. 140∿240 ml of the effluent containing monoguanylmelamine is taken and adjusted to a certain volume by adding 0.1 mol/l sodium hydroxide solution. The absorbance of the solution is measured at 255 nm. This method is applicable for the determination of the samples containing melamine, diguanylmelamine, acetoguanamine, ammeline, dicyandiamide, acetoguanamide, acetoguanide and cyanomelamine.
山梨大学の論文