大腸菌固定化菌体によるグルコースからの水素生成反応に於けるL-スレオニンの作用

概要

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Activation by putting the imobilized E.coli IAM 1264 cells into phosphate buffer solution was affected for the putting time above concentration of the buffer solution. Most effective condition for activating in the range of experiments was putting the imobilized bacterium cells in 1/40 mol phosphate buffer solution (pH 7.5) for an hour. Activity of enzymes of the Embden-Meyerhof-Parnas pathway and pyruvate-formatelyase appeared to be enhanced by this treatment. Addition of L-threonine (0.05g) or 25% ammonia water (0.035ml×3) in the solution for the hydrogen evolution from glucose stimulated to evolve three or two times volume of hydrogen by no use of them. a-ketobutyric acid decomposed of L-threonine or acetoin similar to a-ketobutyric acid as chemical structure caused to 65∿80% evolution of hydrogen from glucose by the addition of L-threonine. But 2,3-butandiol was not so effective as acetoin. And L-threonine was effective for the hydrogen evolution from pyruvate than from formate. It was considered in these facts that L-threonine was broken down to ammonia and a-ketobutyric acid by L-threonine deaminase of the bacterium, then ammonia activated the enzymes of EMP pathway and a-ketobutyric acid stimulated the action of pyruvate-formatelyase to inhibit the activity of enzymes of 2,3-butandiol formation.
東海大学の論文