<研究報告>アオバエのインタクトな唇弁に存在するα-グルコシダーゼ活性の性質

概要

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1. Some properties of α-glucosidase activity detected on the outside of the labellum of the blowfly, Phormia regina Meigen, were examined by "floating method" 2. The enzyme activity on the outside of the labellum was in consistent with single Michaelis-Menten type kinetics for PNPG and sucrose, and the Km values for PNPG and sucrose were 2-8 mM and 100-200 mM, respectively. 3. Activity of the enzyme on the outside of the labellum was little affected by the alteration of pH from 8 to 5 and descreased gradually at lower pH. 4. The enzyme activity on the outside of the labellum is inhibited by Tris in a competitive manner and by Ca^<++> ion, and the K_i value of Tris for the enzyme was about 10 mM. 5. The present results have shown that the enzyme on the outside of the labellum has properties fairly similar to those of the enzyme at the tip of the labellar chemosensory hair. 6. The present results were compalred with those of the α-glucosidase isozymes in the extract from the proboscis, and it became clear that the enzyme in the intact state has properties to distinguish it from the enzyme in the soluble form. 7. Apparent correspondences were observed between the properties of the enzyme on the outside of the labellum and the labellar sugar receptor examined electrophysiologically in all points.
福岡女子大学の論文
1976-03-31