<原著>延長仮骨における骨基質蛋白の遺伝子発現

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概要

• 論文の詳細を見る

• Gene expression of c-fos, osteonectin, osteocalcin and type I , II and III collagens were studied in the lengthened callus made in rabbit tibia and rat femur. The lengthened callus was separated in to two parts histologically ; peripheral subperiosteal callus and central medullary callus. Northern blot analysis revealed that c-fos mRNA was expressed in the peripheral subperiosteal callus earlier and more markedly than those in the central medullary callus, although expression in the medullary callus continued until 40 days after lengthening was completed. In situ hybridization technique revealed that the mRNA of osteonectin was expressed massively in the trabecular portion of the peripheral callus earlier, and expressed in those of central medullary callus later, but was not expressed in the cartilaginous tissue. Immunohistochemically, fos protein was first observed in the large osteoblasts on the matured bony trabeculae in the peripheral periosteal callus, and later, in the osteoblastic cells in primitive bones in the mesenchymal cell layer of the central medullary callus. Osteocalcin was positively stained in the bony matrix and osteocytes were found in the peripheral subperiosteal callus, while osteoblasts were found in the primitive trabeculae in the central medullary callus. Type I collagen appeared in the osteoblasts of the primitive bone trabeculae. Type II collagen was indicated in the perilacunar area of matured cartilaginous tissue. Type III collagen appeared in the immature mesenchymal cells adjacent to the trabecular layer in the eariest stage. These results suggest that gene expression of fos and type III collagen initiated the differentiation of mesenchymal cells in to osteoblasts and chondroblasts, while gene expression of osteonectin and type I collagen stimulated massive primitive bone matrix formation, and gene expression of osteocalcin regulated the calcification and maturation of the bone matrix.

• 近畿大学の論文
• 1994-12-25

著者

• 戸谷 由樹

  近畿大学整形外科

• 戸谷 由樹

  近畿大学医学部附属病院救命救急センター

• 戸谷 由樹

  近畿大学医学部整形外科

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