<原著>レチノイン酸によるラット二分脊椎の発生過程に関する研究
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概要
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Retinoic acid (RA) induces various malformations when given to pregnant rats. The present study was undertaken to elucidate the pathogenesis of spina bifida, one of the major malformations induced by RA in embryos of DA strain rats. RA (all-trans type XX. Sigma) was orally given at a single dose of 60 mg/kg of body weight to pregnant DA rats on day 8,9,10 or 11 of gestation. Examination of 20-day-old fetuses revealed that administration of RA on day 10 of gestation led to malformations involving craniofacial and axial skeletal anomalies. The pathogenesis of spina bifida was then selected for study by light, scanning, and transmission microscopy. The region of presumped spina bifida was examined 3,6,9,12,18 or 24 hours after administration of RA at 60 mg/kg on day 10 of gestation. This series revealed that the round contour of neuroepithelial cells due to disruption of the microtubles, and the rigid continual basal lamina at the basal side of the neuroepithelium accounted for the open neural tube. Twenty four hours after the administration of RA, the neuroepithelium was thickened due to proliferation of neuroepithelial cells. These findings suggested that the primary effects of RA on the neuroepithelial cells leads to an inability of neural folds to elevate due to inhibition of morphological change in the neuroepithelial cells, essentially required for the elevation of the neural fold, and to the rigid continual basal lamina.
- 近畿大学の論文
- 1993-12-25