<原著>骨芽細胞におけるALP活性とその細胞内調節機構 : プロテインキナーゼCの関与および周期的牽引の影響

概要

論文の詳細を見る
Although alkaline phosphatase activity is a marker of osteoblast differentiation and bone formation, related regulatory mechanisms are not well defined. Osteoblast-like cells (HT-3) have protein kinase C and that alkaline phosphatase activity is reduced with activation of protein kinase C. However, alkaline phosphatase activity is also regulated by prostaglandin E2,since this activity is reduced in the presence of this prostaglandin. Since it has been shown clinically that induced micromovement leads to rapid healing of long bone fractures, we investigated the effect of applied cyclic stretch on the alkaline phosphatase activity of HT-3 cells, using a computerized and pressure-operated instrument that physically deforms the cells. As cyclic stretch enhanced alkaline phosphatase activity in the cells, the micromovement seems to have a direct effect on osteoblasts. Cyclic stretch reduced production of prostaglandin E2. It would appear that the stimulatory effect on alkaline phosphatase activity is regulated by a reduction in production of prostaglandin E2.
近畿大学の論文
1993-03-25