<原著>腎孟腎炎惹起性P線毛保有大腸菌に関する研究
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概要
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To determine the growth condition of pyelonephritis associated P-pili (PAP), 11 urinary isolates of the PAP positive Escherichia coli (E. coli) from patients with urinary tract infections (UTI) were cultured. When cultured for forty-eight hours the strains showed the best expression of the PAP adhesion, and when cultured for 24 hours the strains expressed the best piliation, in the Luria broth at 37℃. Further the PAP positive E. coli was screened in 93 urinary strains from patients with UTI and 204 fecal strains from normal mothers of the perinatal period and newborn infants by an immunofluorescent assay (IF) with a monoclonal antibody against the PAP. Fifty strains out of the 52 regarded as PAP positive by hemagglutination (HA), which agglutinated the human erythrocytes of P_1,P^K_1,P^K_2 and did not agglutinate the erythrocytes of human p, bovine and guinea pig, showed positive IF, and none of the other 245 strains showed positive IF. The ratio of the PAP positive E. coli to the total E. coli isolated from urine in acute pyelonephritis, chronic pyelonephritis, acute cystitis, asymptomatic bacteriuria, probable-UTI and the controls was 8/10 (80.0%), 4/12 (33.3%), 3/16 (18.8%), 3/8 (37.5%), 0/9 (0%), and 3/38 (7.9%), respectively, suggesting a pathogenic association of the PAP positive E. coli with UTI. Fecal isolates from the mothers excluding the cases with gestational toxicosis or renal disease, and the newborn infants of 3-5 days old and one month old excluding the cases with hyperbilirubinemia and of low birth weight showed positive IF in the ratio of 11/65 (16.9%), 1/42 (2.3%), and 12/65 (18.5%), respectively. In 44 pairs of the mothers and babies E. coli was isolated both from mothers and their infants of one month old and five pairs out of the 44 had PAP E. coli. These findings indicate that PAP positive E. coli can be transmitted from mothers to their newborn infants during the postnatal period.
- 1989-03-25