<原著>Thy-1^+樹枝状細胞の細胞株樹立

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The phenotype of Thy-1^+ dendritic epidermal cells (Thy-1^+, Lyt5^+, Lyt1^-, Lyt2,L3T4^-, Ia, sIg^-) is different from that of mature peripheral T cells. To get the highly enriched Thy-1^+ dendritic epidermal cell (DEC) fraction for the purpose of corroborating the difference, a method which allows a 30-50 fold enrichment of it was established. With this method, two-step density gradient centrifugation was used. After these steps, monoclonal antibodies (anti-Ia antibody, anti-Lyt1 antibody) and complement were also used to remove the peripheral cells contaminated by leukocytes. The growth requirements for the enriched Thy-1^+ dendritic epidermal cell fraction thus obtained were studied. Con A 5 μg/ml, the combination of Con A and Interleukin 2 (IL2), and also the combination of IL2 and Interleukin 1 (IL1) brought on vigorous proliferation of the Thy-1^+ dendritic epidermal cell enriched fraction. The combination of PMA+IL2 or PMA+IL1 failed to trigger the growth of the Thy-1^+ dendritic epidermal cell enriched fraction. Thy-1^+ dendritic epidermal cell enriched fractions were cultured for about six months with the rat factor which contained Con A and IL2. Upon continuing stimulation with the rat factor, Lyt2^+ cells gradually disappeared and the proliferating cells that exhibited and maintained the essential phenotypical features of resident Thy-1^+DEC (Thy1^+, Lyt5^+, Lyt1^-, Lyt2^-, L3T4), though it expressed IL2 receptors in contrast to this in Thy-1^+DEC in situ. Finally eight clones were established. These cell lines have been now kept in culture over one year without contaminating feeder cells.
近畿大学の論文
1988-06-25

著者

山田秀和
近畿大学医学部奈良病院皮膚科

<原著>Thy-1^+樹枝状細胞の細胞株樹立

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