ペントースの比色定量(第 1 報) : 定量法の比較と改良
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概要
- 論文の詳細を見る
For the determination of pentose by colorimetry, specificity and reproducibility of various methods were examined. It was concluded from these experiments that the methods using ferric chloride-phloroglucinol reagent and aniline-acetic acid reagent were the most suitable. When using pentosan as the sample, determination can be made by the method using ferric chloride-phloroglucinol reagent. Coloration by this method, in comparison with D-xylose, was 74% in D-ribose, 56% in L-arabinose, and 23% in D-glucurone, hexose and methylpentose being below 2%. Pentose can be determined up to 20∿160μg./ml. The known composition of the aniline-acetic acid reagent was modified and the degree of coloration by this method, in comparison with D-xylose, was 80% in D-ribose, 67% in L-arabinose, and 5% in D-galactose and D-glucurone, other hexoses and methylpentose being below 2%. Pentose can be determined up to 10∿80μg./2ml. In both cases, the presence of glucosamine, albumin, and sodium chloride do not interfere in the coloration.
- 共立薬科大学の論文
- 1962-12-20
共立薬科大学 | 論文
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