<原報>Monoamine Oxidase に関する研究(第 3 報) : Radioisotopic Assay によるラット脳内 Monoamine Oxidase 活性測定法の検討
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概要
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In previous works, we have used oxygen electrode method or colorimetry for the assay of monoamine oxidase (MAO) activity. Present investigation was undertaken to find out the best experimental conditions for the assay of rat brain MAO activity in milligram quantities of tissue using the radiochemical method which was originally used for the blood plasma amine oxidase by Otsuka and Kobayashi. Following conditions were successful : (1) Substrate concentration; The highest MAO activity was obtained in the range of (10)^<-3>∿2×(10)^<-3>M tyramine at final concentration. (2) Incubation time; The reaction proceeded linearly for 30 minutes and then it's speed downed gradually. And also, 30 minutes was enough to obtain the sufficient counts of radioactivity. Then, the incubation time was fixed for 30 minutes. (3) Buffer system; The activity of the enzyme incubated in 0.1M Tris-HCl buffer (pH 7.5) was higher than that in 0.1M phosphate buffer (pH 7.5). (4) The following conditions seems most recommendable for the assay of the rat brain MAO activity : rat brain tissue, 1∿10mg (ordinary 5mg); buffer system, 0.1M Tris-HCl buffer (pH 7.5); substrate, (10)^<-3>M tyramine; incubation period, 30 minutes at 37℃.
- 共立薬科大学の論文
- 1978-02-26
共立薬科大学 | 論文
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