<原報>リゾチーム処理 Micrococcus lysodeikticus 細胞壁非透析分画の研究
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The non-dialyzable fraction obtained from cell walls of Micrococcus lysodeikticus by treatment with lysozyme has been purified into three fractions by application of a fractional precipitation method using cetyl pyridinium chloride solution. Each fraction contains D-glucose, N-acetyl D-muramic acid, N-acetyl D-glucosamine, N-acetyl D-muramic acid 6-phosphate and N-acetyl D-glucosamine 6-phosphate as the component carbohydrate, and has the pentapeptide having molar proportions of alanine 2,glutamic acid 1,glycine 1 and lysine 1. The significant differences on the chemical properties of fractions were found in the amounts of glucose, N-acetyl-glucosamine 6-phosphate and the peptide. From the results of the treatments with two phosphatases, a phosphodiesterase and trichloroacetic acid, it is conceivable that the both amino sugar phosphates form phosphodiester linkages between the peptidoglycan, consisted of alternating N-acetylglucosamine and N-acetylmuramyl peptide residue, and the other glucose-rich polymer.
- 共立薬科大学の論文
- 1969-10-20
共立薬科大学 | 論文
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