Selective Brain Hypothermia Protects against Hypoxic-Ischemic Injury in Newborn Rats by Reducing Hydroxyl Radical Production

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We hypothesized that selective brain hypothermia (SBHT) decreases production ofhydroxyl radicals (・OH) induced by hypoxia-ischemia (H-I) and reperfusion andattenuates neuronal damage in neonatal rat brain. Anesthetized 7-day-old rats weredivided into a normothermia (NT) group (n=6) and a SBHT group (n=7) and subjectedto 90-min H-I, followed by a 90-min recovery period. Brain temperature (BT) wasregulated by a water-cooled metallic plate placed under the head. The BT of the SBHTgroup was set at 31.0+/-1.0℃ during the H-I and recovery period. Microdialysis andthe salicylate-trapping method were used to detect ・OH in the striatum. Neuronaldamage was quantified by counting the surviving neurons at 120 hr after reperfusion.The NT group had significant increases in 2,3-dihydroxybenzoic acid (DHBA)(223+/-166%) and 2,5-DHBA (321+/-153%) above baseline levels. The increases in2,3-DHBA (127+/-40%) and 2,5-DHBA (133+/-33%) were significantly lower (p < 0.01)in the SBHT group. The number of surviving neurons was decreased significantly in theNT group but not in the SBHT group. We conclude that SBHT reduces ・OH productionduring H-I and reperfusion and has protective effects against neuronal damage.
神戸大学の論文