Immunohistochemical Detection of Respiratory Syncytial Virus Infection in the Lung of Child Autopsy Cases

概要

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Viral infection in the respiratory tract is suspected in some cases of infant death; however, in most of those cases, routine postmortem examination has been unable to determine a definitive etiology. Using immunohistochemistry with a specific antibody to respiratory syncytial virus (RSV) in paraffin sections, we investigated a possible association of RSV infection with interstitial pneumonia or bronchitis in four child autopsy cases while two adult cases with cytomegalic inclusion disease, pneumocystis carinii pneumonia, or acute interstitial pneumonia were also included as negative control. Immunoreactivity for RSV was detected in one of the 4 child cases; the bronchial and bronchiolar epithelium were immunostained. No immunoreactivity was observed in the two adult cases. Retrospective microscopic examination in routinely stained slides could find no distinctive findings indicating RSV infection in this case as well as the other three cases. Although further evidence, e.g., detection of the viral nucleic acid in specimens, may be needed, the present results suggest that this antibody can be utilized for detection of RSV infection in autopsy samples.
長崎大学の論文