<Original>Preparation and Characterization of the Antibody against the Aldobiouronic acid Unit of Xylan

スポンサーリンク

概要

• 論文の詳細を見る

• この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。The aldobiouronic acid, 2-O-(4-O-methyl-a-D-glucuronic acid)-D-xylose, which was obtained by partial acid hydrolysis of xylan was coupled to methylated bovine serum albumin (BSA). The resulting sugar-protein conjugate was used as an antigen. Antibodies were raised by immunizing a rabbit with the antigen. The specificities for the antiserum obtained were examined by immunodiffusion analysis and enzyme-linked immunosorbent assay (ELISA). These results indicate that the antiserum contains antibodies against both BSA and the antigen. After removal of anti-BSA antibody by affinity chromatography, the specificity of the purified antiserum to wood cell wall polysaccharides and their hydrolysates was investigated by ELISA. The antiserum preferentially reacted with the conjugates containing xylan and aldobiouronic acid, which suggests that the antiserum recognizes the aldobiouronic acid unit of xylan.

• 京都大学の論文
• 1990-12-28

著者

• Sumiya Kazuo

  Wood Research Institute Kyoto University:(present Address)emeritus Professor Kyoto University

• TAKAHASHI Nobuyoshi

  Research Section of Wood Chemistry, WOOD RESEARCH INSTITUTE, KYOTO UNIVERSITY

• Takahashi Nobuyoshi

  Research Section Of Wood Biology Wood Research Institute Kyoto University

関連論文

• Properties of Enzyme-Unhydrolyzable Residue of Lignin-Carbohydrate Complexes Isolated from Beech Wood
• Preparation and Characterization of the Antibody against the Aldobiouronic acid Unit of Xylan

スポンサーリンク