<Originals>An experimental study of peripheral nerve regeneration by utilizing a collagen film comparison with a silicone tubing method
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概要
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The author examined peripheral nerve regeneration in 64 rats in which both sciatic nerves had been severed and the nerve on one side had been treated with collagen film wrapping and that on the other with silicone tubing. Morphological and electrophysiological results for silicone tubing and collagen film wrapping methods were compared. The gap between the proximal and distal stumps of the severed nerve was maintained at a distance of 10 mm. Chronological observation of surviving rats was performed at 1,2,3,4,6,9,12 and 15 months after operation. Morphometric analysis of the number of axons, mean axon diameter, percent large axons, and myelin ratio at the mid position within the gap of the regenerating nerve trunk was performed. Electrophysiological examination and toe-spreading tests were performed to evaluate the recovery of motor function. Severed nerves could be bridged in both experimental models, even though the nerve defect was 10mm in length. Recoverv from conductive failure on the wrapping side was found in about 90% of experimental animals, and in about 65% on the tubing side. Final number of axons was approximately 1,550 on the wrapping side and 1,800 on the tubing side. Mean axon diameter was about 4μm on the wrapping side and about 3.2μm on the tubing side. Mean conduction velocity (MCV) recovered to about 35m/sec on the wrapping side and to about 25m/sec on the tubing side. Remarkable differences in functional recovery and maturation between the tubing and wrapping sides became significant 6 months after operation. Nerve repair with axon maturation and restored motor function was complete within 15 months after operation. Collagen film is an excellent nerve conduit material superior to silicone tubing in inducing recovery of motor function and especially in inducing the maturation of the sprouted nerve axons.
- 近畿大学の論文