<Originals>Biological and Biochemical Study of Escherichia coli Spheroplasts Induced by Penicillin G : Reversion and Chromosomal DNA

概要

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Escherichia coli spheroplasts induced by penicillin G can revert to their parent bacillary forms (parent cells) in an adequate condition, when penicillin G is removed (reversion). Although they continued to synthesize chromosomal deoxyribonucleic acid (DNA) and contained six times the amount of DNA as one parent cell did, de novo DNA synthesis was necessary for the reversion of E. coli spheroplasts to parent cells. Chromosomal DNA of the parent cells was different from that of the spheroplasts judging from sucrose gradient centrifugation and electron microscopy. Two types of chromosomal DNA, a slower and a faster sedimenting form, were released from the gently lysed parent cells. In contrast, the chromosome from the spheroplasts showed a single intermediate value of sedimentation coefficient between those of the chromosomal DNA from the parent cells. The electron microscopy showed that the chromosome of E. coli spheroplasts spread to form an aggregated mass consisting of several chromosome molecules of the parent cells. Moreover, in E. coli spheroplasts, R and F' plasmids were also continuously synthesized. No aggregation of plasmid DNA was observed by electron microscopy.
近畿大学の論文
1984-12-28