<Originals>DNA damage and repair capacity of peripheral mononuclear cells in adult T cell leukemia, hairy cell leukemia and chronic lymphocytic leukemia
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In DNA damage and repair capacity of peripheral mononuclear cells after UV-irradiation adult T cell leukemia, hairy cell leukemia, hairy cell leukemia variant, B-cell type of chronic lymphocytic leukemia and healthy donors were compared. The survival curve of mononuclear cells 24 hr after ultraviolet-irradiation in these leukemia disclosed lower viability than that in normal controls. DNA used was extracted from non-irradiated mononuclear cells, and from mononuclear cells incubated for 0-, 30-, or 60 min after ultraviolet-irradiation. Low repair capacity was demonstrated in these leukemia. The activity of crude DNA polymerase from hairy cell leukemia variant was decreased to 42% of that of the controls. On the priming ability test of extracted double strand DNA for E. coli polymerase I, incorporation of [methyl-^3H]thymidine triphosphate in adult T cell leukemia was higher than that of the controls, and one case of adult T cell leukemia and hairy cell leukemia variant exhibited remarkably increased incorporation. Eluate pattern of hydrolyzed DNA was similar to that of control DNA in liquid chromatography. So, these findings suggested that higher priming ability for DNA polymerase of these patients did not correlate to adenine-thymine/guanine-cytosine ratio. The studies with 5-20% sucrcse density gradient sedimentation revealed that molecular weight of the DNA with higher priming ability was smaller than that of the DNA with lower priming ability, the control DNA being identical in molecular weight to the DNA with lower priming ability. These data suggested that the DNA with high priming ability was a nicked and defected patch. Furthermore, the DNA with higher priming ability was more sensitive to γ-irradiation in vitro, which indicated that these DNAs were shortened to smaller sequence in molecular size. Therefore, these data also supported that the DNA with higher priming ability was a nicked and defected patch.
- 近畿大学の論文
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- DNA damage and repair capacity of peripheral mononuclear cells in adult T cell leukemia, hairy cell leukemia and chronic lymphocytic leukemia