Radical Gas-Based DNA Decontamination for Ultra-Sensitive Molecular Experiments
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概要
- 論文の詳細を見る
In this study, we tested a radical gas-based decontamination technique to prevent possible DNA contamination by the air and/or equipment used in molecular experiments. We prepared 104 molecules of model DNA contaminant and placed the dried DNA into test tubes, which were then exposed to radical gas. Quantitative PCR analysis showed that, even after a short exposure time of 30 minutes, 99.54% of the model DNA contaminant was effectively decomposed to undetectable levels. Our results demonstrate that the radical gas-based treatment is a useful method for eliminating potential DNA contaminant in ultra-sensitive molecular experiments.
- 2012-12-01
著者
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Yamamoto Katsuhiro
Department Of Applied Biochemistry School Of Dairy Science Rakuno Gakuen University
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YAMAMOTO KATSUHIRO
Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology
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MORONO YUKI
Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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INAGAKI FUMIO
Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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