Identification of Material Cultivar of Green Tea Infusions by Simple Sequence Repeat Markers

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概要

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• Due to the recent increase in the consumption of ready-to-drink beverages containing green tea, a method for authenticating the tea cultivar indicated on product label is needed. However, methods for isolating DNA and identifying the cultivar of the green tea used in the beverage have not been reported. We developed a method to identify the cultivar used in green tea infusions using simple sequence repeat (SSR) markers. DNA extracts were obtained by freeze-drying green tea infusions, followed by isopropyl alcohol precipitation, cetyltrimethylammonium bromide extraction, and purification with a commercial kit. Some SSR markers could be amplified using the purified DNA as a template, and peak patterns were matched to patterns from DNA extracted from fresh leaves of cultivars. Application of this method to a commercial green tea beverage identified a monovarietal.

• 社団法人　日本食品科学工学会の論文
• 2012-03-01

著者

• Tanaka Junichi

  Makurazaki Tea Research Station Nivts Naro

• Hayashi Nobuyuki

  Kanaya Tea Research Station National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organization (naro)

• UJIHARA Tomomi

  Kanaya Tea Research Station, National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Organization (NARO)

• Tanaka Junichi

  Makurazaki Tea Research Station Naro Institute Of Vegetable And Tea Science

• UJIHARA Tomomi

  Kanaya Tea Research Station, NARO Institute of Vegetable and Tea Science

関連論文

• Expressed sequence tags from organ-specific cDNA libraries of tea (Camellia sinensis) and polymorphisms and transferability of EST-SSRs across Camellia species
• Construction of a high-density reference linkage map of tea (Camellia sinensis)
• Identification of Material Cultivar of Green Tea Infusions by Simple Sequence Repeat Markers

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