High Cadmium-Binding Ability of a Novel Colocasia esculenta Metallothionein Increases Cadmium Tolerance in Escherichia coli and Tobacco

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概要

• 論文の詳細を見る

• Experimental evidence in vivo as to the functional roles and binding properties to cadmium (Cd) of type-2 plants metallothionein (MT) has been limited thus far. We investigated the biological role of metallothionein from Colocasia esculenta (CeMT2b) in Escherichia coli and tobacco, and developed a new model for the relationship between Cd tolerance and Cd-binding ability. Heterologous expression of CeMT2b in Escherichia coli greatly enhanced Cd tolerance and accumulated Cd content as compared to control cells. The molecular weight of CeMT2b increased with Cd, and CeMT2b bound up to 5.96±1 molar ratio (Cd/protein). Under Cd stress, transgenic tobacco plants displayed much better seedling growth and high Cd accumulation than the wild type. The presence of an extra CXC motif in CeMT2b contributed to the enhanced Cd-tolerance. The present study provides the first insight into the ability of type-2 plant MT to bind physiological Cd.

• 社団法人 日本農芸化学会の論文
• 2011-10-23

著者

• PATEL Darshan

  Bio-energy Research Institute, Chonnam National University

• Patel Darshan

  Bio-energy Research Institute Chonnam National University

• KIM Yeon-Ok

  Bio-Energy Research Institute, Chonnam National University

• LEE Dae-Seok

  Department of Forest Products and Technology (BK21 Program), Chonnam National University

• SONG Younho

  Department of Bioenergy Science and Technology, Chonnam National University

• BAE Hyeun-Jong

  Bio-Energy Research Institute, Chonnam National University

• Lee Dae-seok

  Department Of Forest Products And Technology (bk21 Program) Chonnam National University

• Bae Hyeun-jong

  Bio-energy Research Institute Chonnam National University

• Kim Yeon-ok

  Bio-energy Research Institute Chonnam National University

• Song Younho

  Department Of Bioenergy Science And Technology Chonnam National University

関連論文

• Characterization and application of recombinant β-glucosidase (BglH) from Bacillus licheniformis KCTC 1918
• High Cadmium-Binding Ability of a Novel Colocasia esculenta Metallothionein Increases Cadmium Tolerance in Escherichia coli and Tobacco
• The Transgenic Poplar as an Efficient Bioreactor System for the Production of Xylanase
• Zn tolerance of novel Colocasia esculenta metallothionein and its domains in Escherichia coli and tobacco

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