Bacterial surface Display of a Co-Factor Containing Enzyme, ω-Transaminase from Vibrio fluvialis Using the Bacillus subtilis Spore Display System

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概要

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• To improve the conventional bacterial surface display systems and to display a co-factor containing enzyme, ω-transaminase from Vibrio fluvialis, which needs pyridoxal phosphate (PLP) for efficient transamination, Bacillus subtilis spore display system with cotG, as an anchoring motif was used. Flow cytometry of the B. subtilis spore-expressing ω-transaminase proved its surface localization on the spore. The enzymatic activity of the spore expressing ω-transaminase was more than 30 times higher than that of the host spore. Protease treatment of the ω-transaminase displaying spores resulted in decreased transaminase activity, which is in keeping with the surface location of the fusion protein, CotG-ω-transaminase.

• 社団法人 日本農芸化学会の論文
• 2011-09-23

著者

• Kim June-Hyung

  Department of Chemical Engineering, Dong-A University

• Kim June-hyung

  Department Of Chemical Engineering Dong-a University

• HWANG Bum-Yeol

  Department of Chemical Engineering and Bioengineering and The Helen Wills Neuroscience Institute, Un

• Kim Byung-gee

  Institute For Molecular Biology And Genetics And School Of Chemical Engineering Seoul National Unive

• Hwang Bum-yeol

  Department Of Chemical Engineering And Bioengineering And The Helen Wills Neuroscience Institute Uni

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• High-throughput characterization of lipopolysaccharide-binding proteins using mass spectrometry
• Bacterial Surface Display of a Co-Factor Containing Enzyme, ω-Transaminase from Vibrio fluvialis Using the Bacillus subtilis Spore Display System
• Bacterial surface Display of a Co-Factor Containing Enzyme, ω-Transaminase from Vibrio fluvialis Using the Bacillus subtilis Spore Display System

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