An Efficient and Novel Screening Model for Assessing the Bioactivity of Extracts against Multidrug-Resistant Pseudomonas aeruginosa Using Caenorhabditis elegans

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概要

• 論文の詳細を見る

• As a large number of multidrug-resistant bacteria have emerged, and there is an urgent need for the development of new antibacterial agents. In this study, we developed a liquid-based slow killing assay to be carried out in standard 96-well microtiter plates. This screening method was designed to facilitate high-throughput screening of small molecules and extracts. In antibiotic rescue assays, the Caenorhabditis elegans multidrug-resistant Pseudomonas aeruginosa infection model displayed a high degree of drug resistance in vivo and in vitro. We used the method to screen 1,300 extracts, and found 36 extracts (2.7%) which prolonged the survival of infected nematodes, and four (0.3%) of these extracts showed in vitro and in vivo anti-multidrug resistant P. aeruginosa activity. These results indicate that the whole-animal C. elegans multidrug-resistant bacterial model can be used to screen antibacterial compounds, and can also be useful for bioactive compounds which most likely cannot be identified in vitro.

• 社団法人 日本農芸化学会の論文
• 2011-09-23

著者

• SHAO Lei

  State Key Lab for New Drug and Pharmaceutical Processes, Shanghai Institute of Pharmaceutical Indust

• LI Ji-an

  State Key Lab for New Drug and Pharmaceutical Processes, Shanghai Institute of Pharmaceutical Indust

• HAN Li-zhong

  Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine

• CAI Wai-jiao

  Institute for Integrative Chinese and Western Medicine, Huashan Hospital, Fuden University

• ZHU Chun-bao

  State Key Lab for New Drug and Pharmaceutical Processes, Shanghai Institute of Pharmaceutical Indust

• Chen Dai-jie

  State Key Lab For New Drug And Pharmaceutical Processes Shanghai Institute Of Pharmaceutical Industr

• Zhou Yu-meng

  State Key Lab For New Drug And Pharmaceutical Processes Shanghai Institute Of Pharmaceutical Industr

• Cai Wai-jiao

  Institute For Integrative Chinese And Western Medicine Huashan Hospital Fuden University

• Li Ji-an

  State Key Lab For New Drug And Pharmaceutical Processes Shanghai Institute Of Pharmaceutical Industr

• Han Li-zhong

  Shanghai Ruijin Hospital Shanghai Jiaotong University School Of Medicine

• Zhu Chun-bao

  State Key Lab For New Drug And Pharmaceutical Processes Shanghai Institute Of Pharmaceutical Industr

• Shao Lei

  State Key Lab For New Drug And Pharmaceutical Processes Shanghai Institute Of Pharmaceutical Industr

関連論文

• An Efficient and Novel Screening Model for Assessing the Bioactivity of Extracts against Multidrug-Resistant Pseudomonas aeruginosa Using Caenorhabditis elegans
• An Efficient and Novel Screening Model for Assessing the Bioactivity of Extracts against Multidrug-Resistant Pseudomonas aeruginosa Using Caenorhabditis elegans

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