Grape Seed Procyanidin B2 Inhibits Human Aortic Smooth Muscle Cell Proliferation and Migration Induced by Advanced Glycation End Products
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概要
- 論文の詳細を見る
Advanced glycation end product (AGE)-induced vascular smooth muscle cell (VSMC) proliferation is vital to the progression of diabetic vasculopathy. A grape seed procyanidin extract has been reported to possess anti-oxidative and anti-inflammatory properties and to display a significant cardiovascular protective effect, but little is know about the underlying mechanism. The objective of this present study was to determine whether GSPB2 (grape seed procyanidin B2), which is a dimeric procyanidin and more biologically active, could inhibit AGE-induced VSMC proliferation by affecting the production of ubiquitin COOH-terminal hydrolase 1 (UCH-L1), the degradation of IκB-α and nuclear translocation of NF-κB in human aortic smooth muscle cells (HASMCs). Our data show that GSPB2 preincubation markedly inhibited AGE-induced proliferation and migration of HASMCs in a dose-dependent manner and upregulated the protein level of UCH-L1. Further studies revealed that the GSPB2 pretreatment markedly attenuated the degradation of IκB-α and nuclear translocation of NF-κB by modulating ubiquitination of IκB-α in AGE-exposed HASMCs. These results collectively suggest that AGE-induced HASMC proliferation and migration was suppressed by GSPB2 through regulating UCH-L1 and ubiquitination of IκB-α. GSPB2 may therefore have therapeutic potential in preventing and treating vascular complications of diabetes mellitus.
- 社団法人 日本農芸化学会の論文
- 2011-09-23
著者
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Zhang Zhen
Key Lab Of Drug Metabolism & Pharmacokinetics Beijing Institute Of Pharmacology And Toxicology
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CAI Qian
Key Laboratory of Cardiovascular Proteomics of Shandong Province, Department of Geriatrics, Qi-Lu Ho
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ZHANG Jian-hua
Institute of Basic Science, Medical Science Academy of Shandong
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WANG Jun-fu
Institute of Basic Science, Medical Science Academy of Shandong
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YIN Mei
Key Laboratory of Cardiovascular Proteomics of Shandong Province, Department of Geriatrics, Qi-Lu Ho
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Li Bao-ying
Key Lab. Of Cardiovascular Proteomics Of Shandong Province Dep. Of Geriatrics Qi-lu Hospital Of Shan
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Gao Hai-qing
Key Lab. Of Cardiovascular Proteomics Of Shandong Province Dep. Of Geriatrics Qi-lu Hospital Of Shan
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Yu Fei
Key Laboratory Of Cardiovascular Proteomics Of Shandong Province Department Of Geriatrics Qi-lu Hosp
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Wang Jun-fu
Institute Of Basic Science Medical Science Academy Of Shandong
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Zhang Jian-hua
Institute Of Basic Science Medical Science Academy Of Shandong
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Li Bao-ying
Key Laboratory Of Cardiovascular Proteomics Of Shandong Province Department Of Geriatrics Qi-lu Hosp
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Yin Mei
Key Laboratory Of Cardiovascular Proteomics Of Shandong Province Department Of Geriatrics Qi-lu Hosp
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Gao Hai-qing
Key Laboratory Of Cardiovascular Proteomics Of Shandong Province Department Of Geriatrics Qi-lu Hosp
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Zhang Zhen
Key Laboratory Of Cardiovascular Proteomics Of Shandong Province Department Of Geriatrics Qi-lu Hosp
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Cai Qian
Key Laboratory Of Cardiovascular Proteomics Of Shandong Province Department Of Geriatrics Qi-lu Hosp
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- Grape Seed Procyanidin B2 Inhibits Human Aortic Smooth Muscle Cell Proliferation and Migration Induced by Advanced Glycation End Products
- Grape Seed Procyanidin B2 Inhibits Human Aortic Smooth Muscle Cell Proliferation and Migration Induced by Advanced Glycation End Products