リボソームタンパク質をバイオマーカーとしたマトリックス支援レーザー脱離イオン化質量分析法によるバクテリアの分類・同定法の開発

概要

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A rapid and reliable method for the identification and phylogenetic classification of bacteria is described. In recent years, a method for the rapid identification of bacteria at the “species level” using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) were proposed. In this method, observed mass spectral peaks, which are used as biomarkers, are assigned using the calculated masses of ribosomal subunit proteins based on amino acid sequences registered in protein databases. We applied the proposed method to a variety of samples. including genome sequenced bacteria, and found that the method is reliable and practical for rapidly identifying bacteria at the “species level.” In addition, minor sequence variations in ribosomal subunit proteins at the “strain level” can be clearly detected as mass differences on MALDI mass spectra. Using the mass differences, it was possible to implement a method for the rapid and reliable phylogenetic classification of bacteria at the “strain level.” The method was also compared with analogous classifications using gene analysis.
2011-10-01